Featured Member Archives


Meet Travis Doggett, Ph.D. recipient of the 2016 August Krogh Young Investigator Award
 

The current research interest of Dr. Doggett (University of South Florida) focuses on microvascular permeability following hemorrhagic shock/trauma combined with acute alcohol intoxication. Binge drinking is a serious problem in the United States, producing $223.5 billion in healthcare costs. Nearly 40% of injured patients admitted to the emergency room have intoxicating blood alcohol concentrations. Intoxicated trauma patients exhibit aggravated hemodynamic instability following hemorrhage, are significantly more hypotensive, and require greater volumes of resuscitation fluids compared to non-intoxicated patients. His lab has demonstrated that acute alcohol intoxication causes microvascular hyperpermeability in the mesenteric microcirculation of rats. More recent work has investigated microvascular permeability following a fixed-pressure hemorrhage combined with acute alcohol intoxication. We hypothesize that the loss of central fluid volume due to elevated hyperpermeability is directly responsible for the potentiated hypotension exhibited by intoxicated trauma patients. Currently, we are investigating methods to enhance the microvascular barrier and attenuate hyperpermeability following hemorrhage. The August Krogh Young Investigator Award is named in honor of microcirculatory scientist August Krogh, who received the Nobel Prize in 1920 for the first descriptions of blood flow control through capillaries and arterioles based on tissue oxygen demand, this annual award is intended for a graduate student or a Ph.D./M.D. in the early stages of a research career. This award is presented to encourage excellence in microcirculatory research by new, young investigators.

Meet Zhichao Fan, Ph.D. of the La Jolla Institute for Allergy and Immunology
 

One of Dr. Fan’s research interests is focusing on studying the mechanisms of leukocyte rolling and arrest during inflammation in the microcirculation. Dr. Fan exploited microfluidics with molecularly defined surfaces and high resolution three-color total internal reflection microscopy to imaging the molecular dynamics during leukocyte rolling and arrest under high resolution in vitro, and using intravital microscopy to study leukocyte rolling and arrest in vivo. Adhesion molecules such as integrins were mainly focused.

Another research interest of Dr. Fan, is monitoring rare circulating cells, such as circulating tumor cells, in microcirculation using in vivo flow cytometry.

 

Dr. Steven Segal Named the 2016 Landis Awardee

Professor Steven Segal received his B.A. and M.A. degrees in Physical Education and Exercise Physiology from the University of California at Berkeley followed by a dual Ph.D. in Kinesiology and Physiology from the University of Michigan. He then undertook postdoctoral training at the University of Virginia in the laboratory of Dr. Brian Duling. After serving on the faculty of the Noll Human Performance Laboratory at Pennsylvania State University, Dr. Segal moved to Yale School of Medicine where he rose through the academic ranks to tenured Professor in Cellular and Molecular Physiology. He then moved to the Department of Medical Pharmacology and Physiology at the University of Missouri-Columbia, where he currently holds the title of Margaret Proctor Mulligan Professor in Medical Research. Dr. Segal is also an Investigator in the Dalton Cardiovascular Research Center.

With over three decades in microcirculation research, Professor Segal has built an international reputation for his foundational work on the local control of blood flow, particularly with respect to the roles of microvascular endothelium, vascular smooth muscle and perivascular nerves in the moment-to-moment control of blood flow in resistance networks. His work on underlying mechanisms and functional consequences of cell-to-cell communication in the vascular wall has provided new insight into our understanding of how local blood flow is coupled to tissue metabolic demand. Dr. Segal's work is valued by his peers for its rigor and technical innovation. Over the years his laboratory has developed powerful new approaches to resolve the cellular and subcellular processes involved in the microvascular control of blood flow and oxygen delivery with an emphasis on skeletal muscle during exercise.

Professor Segal is the author/co-author of over 100 peer-reviewed articles and more than 20 invited reviews and book chapters. He serves as Reviewing Editor for the Journal of Physiology and has served as Associate Editor for Microcirculation. He is on the editorial board of several other leading journals, including the American Journal of Physiology: Heart and Circulatory Physiology and the Journal of Vascular Research.

Professor Segal has also served on numerous study sections and review groups for the NIH and other funding agencies. His own research has been funded continuously by the NIH for over 30 years, culminating in a prestigious MERIT Award that extends into 2019. Throughout his career, Dr. Segal has been an active and engaged member of scientific societies. He served the Microcirculatory Society in several capacities including President, as a member of Executive Council and as a member and chair of MCS committees. Dr. Segal is also an Established Investigator of the American Heart Association, a Fellow of the American College of Sports Medicine, a Fellow of the AHA Council on Basic Cardiovascular Sciences, and a Fellow of the American Physiological Society Cardiovascular Section. Dr. Segal has received prestigious scientific awards, including the MCS Outstanding Young Investigator Travel Award, the Abbott Microcirculation Award from the European Society for Microcirculation, and the Malpighi award, also from the ESM. Professor Segal's dedication to microvascular research is embodied in his mentoring of the students and postdoctoral fellows who have in turn become successful independent investigators, academicians and clinicians.

D. Neil Granger, 2015 Benjamin W. Zweifach Award Recipient

Dr. D. Neil Granger received his B.S. in Microbiology from the University of Southwestern Louisiana, and an M.S. and Ph.D. in Physiology and Biophysics from the University Of Mississippi School Of Medicine. After rising through the academic ranks at the University of South Alabama, he moved to LSU Health Sciences Center in 1986 to assume his current position as Boyd Professor and Head of the Department of Molecular and Cellular Physiology. Over the last four decades, Dr. Granger has built an international reputation as an innovative scientist and scholar whose groundbreaking research has provided valuable insight into not only the normal regulation of blood flow and microvessel permeability, but also inflammatory and prothrombogenic responses of the microcirculation in ischemia/reperfusion, hypercholesterolemia, obesity, stroke and inflammatory bowel disease. Dr. Granger's work in these areas has had a major impact on multiple disciplines, including physiology, pathology and immunology. He is the author of over 600 peer-reviewed articles, over 100 book chapters and 7 books. Dr. Granger has also served as founding Editor-in-Chief of the Microcirculatory Society's journal Microcirculation, as Associate Editor for the American Journal of Physiology (Gastrointestinal and Liver Physiology) and the journals Microcirculation and Pathophysiology, and on the editorial boards of over a dozen other journals. He has also served on more than 30 study sections and review groups for the NIH and other funding agencies, and his own research has been continuously funded by the NIH for over 30 years. Over the course of his career, Dr. Granger has been active in the leadership of numerous scientific societies, including serving on the Council of the Microcirculatory Society and as its President. He also served as the 77th President of the American Physiological Society (APS). Dr. Granger has received numerous prestigious awards for his scientific achievements, including the APS Bowditch Award, the Distinguished Research Award from the GI Section of the APS, the Landis Award from the Microcirculatory Society, the Dolph Adams Award from the Society for Leukocyte Biology, the Career of Distinction Award from the Oxygen Society, the Nishimaru-Tsuchiya International Award from the Japanese Society for Microcirculation, and the Robert Berne Award from the Cardiovascular Section of the APS. A tireless proponent for microvascular research, Dr. Granger's profound impact on the field extends beyond his own work to include the many students and fellows he has trained who have gone on to become scientific leaders in their own right.

Cam Ha Tran, University of Calgary

Over the past two and half years Dr. Cam Ha Tran has proven to be an outstanding postdoctoral fellow in the laboratory of Dr. Grant Gordon of the University of Calgary. Through an incredible effort that only top trainees can accomplish, she successfully pioneered a highly novel and exciting technique: fully awake mouse in vivo two-photon imaging of the cerebral microcirculation, a ground-up endeavor, as there are only a handful of labs using similar methods internationally. The method she arrived at was published as a large and detailed protocol paper so that other labs may benefit from her pursuits.

In addition, she had the idea to implement a tail artery cannula in the mouse to perfuse agents of interest, plus a fluorescent dye, into the systemic circulation so that we might image the arrival of drugs to the brain. This technique works surprisingly well and is part of her published protocol paper.

Cam Ha's first publication as a first author (co-authored by Dr. Gordon) was published in Frontiers Cellular Neuroscience. Her manuscript detailed how one can achieve sub-cellular level imaging of the brain of fully behaving mice, while also minimizing animal stress. Cam Ha demonstrates how behavioral data can be captured simultaneously with two-photon fluorescence signals. She also shows other possible applications of this technique by 1) monitoring dynamic changes to blood flow in response to sensory stimulation and 2) measuring Ca2+ signals from synthetic and genetically encoded Ca2+ indicators in astrocytes. The method she developed will facilitate acute two-photon fluorescence imaging in awake, active mice and help link cellular events within the brain's microcirculation to whole animal behavior.

In another project, she demonstrates the role of astrocytes in brain blood flow control. In vitro data clearly shows that Ca2+ elevations in astrocytes influence blood vessel diameter, yet in vivo data fails to observe such astrocyte Ca2+ signals during functional increases in blood flow. Notably, the in vivo data is collected under anesthesia or sedation, which reduces astrocyte Ca2+ signaling. Cam Ha's data clearly shows robust spontaneous and evoked astrocyte Ca2+ signals in her awake mouse preparation.
Kwangseok Hong, University of Missouri-Columbia

"The contractile activity of vascular smooth muscle cells is critical to the ability of small arteries to regulate blood flow in response to fluctuations in intraluminal pressure (the "Myogenic Response")." In regard to this, I am interested in the mechanotransduction processes which underlie how smooth muscle cells sense and respond to mechanical forces. In particular, whether the angiotensin II type 1 receptor (AT1R) can act as a mechanosensor in resistance arterioles and contribute to myogenic responsiveness. An additional interest relates to whether pressure-dependent vasoconstriction is modulated by negative regulation of AT1R signaling by RGS (Regulators of G-protein Signaling) proteins under physiological and pathophysiological conditions."   Kwangseok is a recipient of a 2015 MCS Zweifach Student Travel Award.

Evandro M. Neto Neves, Indiana University

Dr. Neves is a Postdoctoral fellow in Emergency Medicine at Indiana University in the lab of Dr. Jeff Kline, Professor of Emergency Medicine and Physiology. His current research interests focus on the study of cardiovascular diseases, including pulmonary embolism (PE) and right ventricular dysfunction. His main hypothesis is that activated proteases, such as matrix metalloproteinases (MMPs) may contribute for the right ventricular and lung vasculature damaged following PE. Interestingly, he and his colleagues have found that doxycycline (an MMP inhibitor) attenuates PE-induced pulmonary hypertension and also protects the heart against damage (prevents troponin I release and right ventricular dilation). In addition, he believes that MMP inhibition could be a useful strategy to decrease mortality and morbidity after PE in the future. Dr. Neves is a recipient of a 2015 MCS Pappenheimer Travel Award.

Kerri-Ann Norton, Ph.D., Johns Hopkins School of Medicine

Dr. Norton is a postdoctoral fellow in the Department of Biomedical Engineering at Johns Hopkins School of Medicine; she is part of the Systems Biology Laboratory under the mentorship of Dr. Aleksander Popel, Professor of Biomedical Engineering, Chemical and Molecular Engineering and Mechanical Engineering. Her graduate studies were completed at Rutgers, the State University of New Jersey, in Computational Biology and Molecular Biophysics, where she developed agent-based models of ductal carcinoma in situ (DCIS) progression and developed 3D reconstructions of human DCIS samples. Dr. Norton's current research is in computational modeling and experimental quantitative flow cytometry to study heterogeneity in breast cancer and its microenvironment, with a particular focus on tumor microvasculature and cell surface receptor composition. She has received several awards for her work such as the American Cancer Society Postdoctoral Fellowship and an NIH training grant in Nanotechnology for Cancer Medicine Fellowship.  Dr. Norton is a recipient of a 2015 MCS Pappenheimer Travel Award.